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Category:Windows boot disksThe use of recombinant tissue plasminogen activator for pulmonary thromboembolism in a prehospital setting.
Pulmonary thromboembolism (PTE) has a significant morbidity and mortality. The administration of thrombolytic agents is the current mainstay of treatment. However, the use of thrombolytics in the prehospital setting has not been described. The objective of this retrospective chart review was to determine the efficacy and safety of a prehospital thrombolytic protocol for PTE in a Level I Trauma Center. Data collected included time to initiation of thrombolytic therapy, time to resolution of thrombolytic therapy, time to hospital arrival, and patient outcome. Thirteen patients received thrombolytic therapy for PTE. The time to thrombolytic therapy was 2.1 +/- 1.2 hours, and time to resolution of thrombolytic therapy was 5.0 +/- 2.3 hours. All patients improved clinically. No patients were admitted to the hospital because of bleeding complications. The current protocol allows for a “front door” thrombolytic therapy protocol in the prehospital setting and demonstrates its efficacy and safety in the management of PTE.A spermatogenic cell factor extracted from seminiferous tubule fluid is a gamma-interferon.
We investigated the possibility that a spermatogenic cell factor present in seminiferous tubule fluid, which is active in an interferon assay, is a gamma-interferon. On the basis of molecular weight and stability studies, we developed an assays for identifying gamma-interferon by radioimmunoassay and enzyme-linked immunosorbent assay. We studied the purity, stability, and presence of protein sequence homology in recombinant and natural gamma-interferons. Moreover, we developed a highly sensitive and specific radioimmunoassay for gamma-interferon and then examined an extract of seminiferous tubule fluid for protein sequence homology to natural and recombinant gamma-interferons. The extract was purified by gel permeation chromatography and anion-exchange and gel filtration chromatography. The use of antibodies raised to gamma-interferon for assaying the material